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. 2020 Sep 1;70(5):915–927. doi: 10.1136/gutjnl-2019-320430

Figure 6.

Figure 6

Escherichia coli is a major contributor to mortality in Western-type diet (WD)+acute necrotising pancreatitis (ANP) mice. (A) Experimental design for gut depletion of E. coli. Gram-negative gut depletion (GNGD) was performed two times per day by oral gavage with non-absorbable antibiotics (neomycin 100 mg/L and polymyxin B 10 mg/L). (B) Kaplan-Meier survival curves demonstrating cumulative survival between treatment groups WD+ANP (n=14) and WD+GNGD+ ANP (n=6). The survival was significantly improved with GNGD (p=0.048, log-rank test). (C) Depletion of Escherichia/Shigella in cecum mucosal tissue in five out of six WD+GNGD+ ANP mice, based on 16S rRNA analysis (p=0.132 by Mann-Whitney). (C and D) Culture analysis demonstrates reduction of E.coli bacterial load in blood. Significant differences were found for incidence (n=6, 0=0.007 by Fisher exact test) and CFU (p=0.006 by Mann-Whitney test) of Gram-negative cultures, but not for Gram-positive. *p<0.05; **p<0.01; ns, not statistically significant.