Figure 7.

Faecal microbial transplant (FMT) in mice following acute necrotising pancreatitis (ANP) exacerbates bacterial dissemination and worsens survival. (A) Experimental design. Mice fed a Western-type diet (WD) for 2 weeks received either FMT (from healthy mice), sterile faecal filtrate (SFF) or vehicle (VEH) by oral gavage at 0, 24 and 48 hours after ANP induction. (B) Kaplan-Meijer survival curves demonstrating significantly attenuated survival with WD +ANP+ FMT compared with control mice that received SFF (WD +ANP+ SFF) (p=0.047 by log-rank test). (C) Total colony forming units (CFU) were increased in the WD +ANP+ FMT group (p=0.024 by Mann-Whitney test). (D) Incidence of Gram-positive and Gram-negative culture positivity in the pancreas. No significant differences were observed (Fisher exact test). (E) Incidence of cultured bacterial species in pancreatic tissue. *p<0.05.