TABLE 3.
Stability of candidate RGs in EVs from different cell types.
| Source | Ranking | Geomean | Delta Ct | BestKeeper | NormFinder | geNorm | |||||
| CCs | 1 | miR-103a-3p | 1.78 | miR-22-5p | 0.42 | miR-23a-3p | 0.10 | miR-660-5p | 0.02 | miR-22-5p | miR-103a-3p | 0.04 |
| 2 | miR-22-5p | 2.00 | miR-103a-3p | 0.42 | miR-103a-3p | 0.10 | miR-16-5p | 0.04 | |||
| 3 | miR-23a-3p | 3.46 | miR-16-5p | 0.43 | let-7a-5p | 0.11 | miR-425-5p | 0.11 | miR-23a-3p | 0.06 | |
| 4 | miR-16-5p | 3.98 | miR-23a-3p | 0.45 | miR-22-5p | 0.12 | miR-22-5p | 0.18 | let-7a-5p | 0.09 | |
| 5 | miR-660-5p | 4.28 | miR-425-5p | 0.45 | miR-221-3p | 0.17 | miR-103a-3p | 0.19 | miR-425-5p | 0.18 | |
| 6 | miR-425-5p | 4.61 | miR-660-5p | 0.46 | miR-425-5p | 0.25 | miR-23a-3p | 0.27 | miR-16-5p | 0.21 | |
| 7 | let-7a-5p | 5.09 | let-7a-5p | 0.49 | miR-16-5p | 0.26 | miR-29a-5p | 0.30 | miR-660-5p | 0.24 | |
| 8 | miR-221-3p | 7.75 | miR-29a-5p | 0.57 | miR-660-5p | 0.32 | let-7a-5p | 0.36 | miR-221-3p | 0.30 | |
| 9 | miR-29a-5p | 8.21 | miR-221-3p | 0.64 | miR-29a-5p | 0.35 | miR-26a-5p | 0.47 | miR-29a-5p | 0.34 | |
| 10 | miR-26a-5p | 9.97 | miR-26a-5p | 0.66 | miR-423-5p | 0.40 | miR-221-3p | 0.51 | miR-26a-5p | 0.39 | |
| 11 | miR-423-5p | 10.74 | miR-423-5p | 0.78 | miR-26a-5p | 0.54 | miR-423-5p | 0.74 | miR-423-5p | 0.44 | |
| 12 | miR-101-3p | 12.00 | miR-101-3p | 1.38 | miR-101-3p | 1.00 | miR-101-3p | 1.37 | miR-101-3p | 0.60 | |
| BMSCs | 1 | miR-103a-3p | 1.57 | miR-103a-3p | 0.38 | miR-103a-3p | 0.07 | miR-29a-5p | 0.03 | miR-22-5p | miR-29a-5p | 0.07 |
| 2 | miR-29a-5p | 1.57 | miR-29a-5p | 0.39 | miR-423-5p | 0.12 | miR-103a-3p | 0.05 | |||
| 3 | miR-22-5p | 2.45 | miR-22-5p | 0.41 | miR-29a-5p | 0.14 | miR-22-5p | 0.17 | miR-103a-3p | 0.12 | |
| 4 | miR-423-5p | 3.36 | miR-423-5p | 0.42 | miR-22-5p | 0.18 | miR-423-5p | 0.24 | miR-423-5p | 0.15 | |
| 5 | miR-23a-3p | 6.12 | miR-23a-3p | 0.45 | miR-221-3p | 0.20 | miR-23a-3p | 0.24 | miR-425-5p | 0.25 | |
| 6 | miR-660-5p | 6.64 | miR-660-5p | 0.49 | miR-660-5p | 0.25 | miR-660-5p | 0.29 | let-7a-5p | 0.27 | |
| 7 | miR-221-3p | 6.88 | miR-16-5p | 0.51 | miR-23a-3p | 0.28 | miR-16-5p | 0.40 | miR-221-3p | 0.30 | |
| 8 | miR-425-5p | 7.54 | miR-221-3p | 0.58 | miR-425-5p | 0.33 | miR-221-3p | 0.48 | miR-23a-3p | 0.36 | |
| 9 | miR-16-5p | 8.37 | miR-425-5p | 0.60 | let-7a-5p | 0.34 | miR-425-5p | 0.56 | miR-660-5p | 0.40 | |
| 10 | let-7a-5p | 8.57 | let-7a-5p | 0.61 | miR-16-5p | 0.36 | let-7a-5p | 0.57 | miR-16-5p | 0.43 | |
| 11 | miR-26a-5p | 11.00 | miR-26a-5p | 0.64 | miR-26a-5p | 0.51 | miR-26a-5p | 0.60 | miR-26a-5p | 0.48 | |
| 12 | miR-101-3p | 12.00 | miR-101-3p | 0.67 | miR-101-3p | 0.53 | miR-101-3p | 0.65 | miR-101-3p | 0.51 | |
| ASCs | 1 | miR-103a-3p | 1.41 | miR-103a-3p | 0.43 | miR-423-5p | 0.11 | miR-103a-3p | 0.09 | miR-23a-3p | miR-103a-3p | 0.10 |
| 2 | miR-23a-3p | 1.68 | miR-23a-3p | 0.45 | miR-23a-3p | 0.14 | miR-23a-3p | 0.19 | |||
| 3 | miR-423-5p | 3.46 | let-7a-5p | 0.47 | miR-221-3p | 0.14 | let-7a-5p | 0.19 | miR-221-3p | 0.21 | |
| 4 | let-7a-5p | 4.05 | miR-26a-5p | 0.51 | miR-103a-3p | 0.21 | miR-26a-5p | 0.28 | miR-423-5p | 0.27 | |
| 5 | miR-221-3p | 5.20 | miR-16-5p | 0.53 | miR-16-5p | 0.33 | miR-16-5p | 0.33 | let-7a-5p | 0.33 | |
| 6 | miR-16-5p | 5.23 | miR-423-5p | 0.54 | let-7a-5p | 0.38 | miR-423-5p | 0.36 | miR-16-5p | 0.37 | |
| 7 | miR-26a-5p | 5.29 | miR-22-5p | 0.57 | miR-26a-5p | 0.39 | miR-22-5p | 0.41 | miR-26a-5p | 0.40 | |
| 8 | miR-22-5p | 8.15 | miR-29a-5p | 0.59 | miR-425-5p | 0.40 | miR-29a-5p | 0.46 | miR-29a-5p | 0.43 | |
| 9 | miR-29a-5p | 8.24 | miR-221-3p | 0.59 | miR-29a-5p | 0.42 | miR-221-3p | 0.46 | miR-22-5p | 0.45 | |
| 10 | miR-660-5p | 10.24 | miR-660-5p | 0.72 | miR-22-5p | 0.55 | miR-660-5p | 0.64 | miR-660-5p | 0.49 | |
| 11 | miR-425-5p | 10.84 | miR-101-3p | 0.76 | miR-660-5p | 0.59 | miR-101-3p | 0.67 | miR-101-3p | 0.53 | |
| 12 | miR-101-3p | 11.24 | miR-425-5p | 0.82 | miR-101-3p | 0.75 | miR-425-5p | 0.76 | miR-425-5p | 0.58 | |
| MSCs* | 1 | miR-103a-3p | 1.41 | miR-103a-3p | 0.51 | miR-423-5p | 0.26 | miR-103a-3p | 0.11 | miR-29a-5p | miR-103a-3p | 0.23 |
| 2 | miR-23a-3p | 2.45 | miR-23a-3p | 0.54 | miR-221-3p | 0.27 | miR-23a-3p | 0.15 | |||
| 3 | miR-423-5p | 2.45 | miR-423-5p | 0.57 | miR-23a-3p | 0.34 | miR-423-5p | 0.25 | miR-23a-3p | 0.26 | |
| 4 | miR-29a-5p | 3.50 | miR-22-5p | 0.59 | miR-103a-3p | 0.36 | miR-22-5p | 0.25 | miR-423-5p | 0.32 | |
| 5 | miR-221-3p | 4.36 | miR-29a-5p | 0.59 | miR-425-5p | 0.38 | miR-29a-5p | 0.31 | miR-221-3p | 0.34 | |
| 6 | miR-22-5p | 5.26 | miR-221-3p | 0.64 | miR-29a-5p | 0.43 | miR-221-3p | 0.40 | miR-22-5p | 0.39 | |
| 7 | miR-26a-5p | 7.24 | miR-26a-5p | 0.67 | miR-26a-5p | 0.45 | miR-26a-5p | 0.46 | miR-660-5p | 0.43 | |
| 8 | miR-660-5p | 8.18 | miR-660-5p | 0.71 | miR-22-5p | 0.46 | miR-660-5p | 0.50 | miR-26a-5p | 0.47 | |
| 9 | miR-425-5p | 8.41 | miR-101-3p | 0.77 | miR-16-5p | 0.47 | miR-101-3p | 0.61 | miR-101-3p | 0.52 | |
| 10 | miR-101-3p | 9.46 | miR-425-5p | 0.81 | miR-660-5p | 0.57 | miR-425-5p | 0.66 | miR-425-5p | 0.57 | |
| 11 | miR-16-5p | 10.46 | miR-16-5p | 0.95 | miR-101-3p | 0.64 | miR-16-5p | 0.86 | miR-16-5p | 0.62 | |
| 12 | let-7a-5p | 12.00 | let-7a-5p | 1.17 | let-7a-5p | 1.16 | let-7a-5p | 1.11 | let-7a-5p | 0.71 | |
Data from three different donors for each cell type were analyzed together to generate a miRNA stability ranking. *RG of BMSCs and ASCs scored together.