FIGURE 5.

Protoplasts isolated from the leaf base and young leaves of maize and rice. (A) Ten-days-old maize and rice seedlings were used for protoplast isolation. (B) The leaf base and young leaves of maize and rice were collected for protoplast isolation, respectively. (C) Protoplasts were successfully isolated from the leaf base of maize and rice, which was better than that of isolated from the young leaves. (D) Viability test of protoplasts isolated from maize and rice using FDA staining. (E) Different D-mannitol concentrations in the enzyme solutions (0.2, 0.3, 0.4, 0.5, 0.6, and 0.7 M) were established for protoplast isolation. Data presented as means of three biological replicates with error bars indicating the SD values, and different letters (a–e) among treatments indicate statically significant differences at p < 0.05 based on Duncan’s multiple range test.