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. 2021 Feb 15;12:626015. doi: 10.3389/fpls.2021.626015

FIGURE 6.

FIGURE 6

Transient expression and protein subcellular localization studies using Cymbidium leaf base protoplasts. (A) Transient expression of GFP in the Cymbidium leaf base protoplasts transfected with empty control vector pAN580-GFP (expressing GFP). (B) Empty control vector, endoplasmic reticulum marker AtWAK2-GFP, plasma membrane marker AtPIP2A-GFP, and CsDELLA-GFP vectors were transformed into Cymbidium protoplasts to test the feasibility of the PTES for protein subcellular localization. Bar, 10 μm.