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The isolated circHIPK3 is circular structure that is located in the cytoplasm. (A) Treated the isolated circHIPK3 with RNase R, circHIPK3 is more resistant to RNase R than linear HIPK3 RNA. (B) Subcellular location detection showed that circHIPK3 was mainly located in cytoplasm. (C,D) RT-PCR analysis of circHIPK3 expression levels in HASMC and HUASMC after transfecting with si-circHIPK3. *P < 0.05.
