Figure 5.

Longitudinal CAP257 54wpi_D-specific systemic and mucosal binding antibodies. Plasma samples from vaccinated macaques were tested longitudinally by ELISA for IgG binding antibodies to (A) autologous CAP257 54wpi_D and (B) heterologous HIV-1 SF162 gp140 trimeric proteins. Correlative analysis between the responses to autologous and heterologous Envs in the DNA+Protein group (C) and the SAd7+Protein group (D). (E) Buccal secretions were tested for IgG content by ELISA against CAP257 54wpi_D gp140 protein after the second and third immunizations (Week 6 and Week 14, respectively) for IgG binding antibodies to CAP257 54wpi_D gp140 trimeric protein. Correlative analysis between the titers of immunogen-specific IgG in the plasma of animals at the time of challenge and IgG present in buccal secretions after the third immunization in the Sad7+Protein group (F) and the DNA+Protein group (G). DNA+Protein group is in blue and SAd7+Protein group is in red, NHPs are identified by individual symbols.