FIG. 5.

Restoration of coordinated neutrophil swarming response to C. albicans following priming with G‐CSF and GM‐CSF. Neutrophils from patients with cirrhosis were primed for 30 minutes with 0.2 ng/mL GM‐CSF, 300 ng/mL G‐CSF, or placebo before being added to C. albicans arrays to examine neutrophil swarming responses. (A) Time to escape of C. albicans hyphae from the swarm. (B) Normalized area of fungal growth by C. albicans by 16 hours comparing primed to untreated neutrophils from patients with cirrhosis: n = 16 swarms for each cirrhotic condition, for hyphal escape; and n = 96 swarms for each cirrhotic condition, for fungal growth, except for C5 GM‐CSF, which has 48 swarms. The average control value is shown as a reference in each graph. ****P ≤ 0.0001 (one‐way analysis of variance with Tukey’s post‐hoc test for (A), and Kruskal‐Wallis with Dunn’s posttest for (B). Error bars represent SD.