FIG. 1.

In vitro models for FADS1‐KD and overexpression by lentiviral vectors (*P < 0.05; **P < 0.01; ***P < 0.001). (A) Western blotting of FADS1 protein level in HepG2 (before and after short hairpin RNA treatment) and Huh7. (B) ORO extract absorbance normalized with mean DAPI measurement in control and FADS1‐KD HepG2 cells in medium supplemented with vehicle, low, medium, and high PA/OA for 24 hours. Statistical analysis is used to compare the ORO reading between FADS1‐KD cells and control cells. (C) ORO reading of Huh7 cells with or without FADS1 overexpression. (D) ORO reading of human primary hepatocytes with or without FADS1‐KD or FADS1‐KD with high PA/OA + 25 μM DHA treatment for 36 hours. Data of a representative experiment (in triplicates) of three independent performances are shown. Abbreviations: Cont., control; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NT, vehicle; OverExp, overexpression; shRNA, short hairpin RNA.