TABLE 1.

Integrated PUFA Lipid Levels in Phospholipids* and Key Lipid Ratios in Control, FADS1‐KD (shRNA), and FADS1 Overexpressing Cells

Lipid/Ratio	Cont. shRNA		FADS1 shRNA		FADS1 OverExp		P Value
	Mean	SD	Mean	SD	Mean	SD	Cont. vs. FADS1 shRNA	FADS1 shRNA vs. OverExp
DGLA	0.69	0.04	0.74	0.02	0.23	0.05	0.03	<0.001
AA	4.14	0.22	2.56	0.06	3.11	0.08	<0.001	<0.001
EPA	0.49	0.04	0.27	0.02	0.25	0.01	<0.001	0.03
DHA	2.43	0.25	1.88	0.06	1.81	0.04	<0.01	NS
AA/DGLA	6.03	0.03	3.43	0.03	14.19	2.47	<0.001	<0.001
EPA/AA	0.12	0.00	0.10	0.00	0.08	0.00	<0.01	<0.001

Data are presented as mean ± SD of four replicates.

^*Mol percentage of total phospholipid.

Abbreviation: NS, not significant.