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. 2021 Feb 4;11:626820. doi: 10.3389/fimmu.2020.626820

Figure 5.

Figure 5

Soluble CD52 inhibits B cell receptor signaling. (A) Calcium flux measurement in human B cells pre-incubated with either Fc (20 µg/ml) or CD52-Fc (20 µg/ml) prior stimulation with anti-IgM (25 µg/ml) (indicated timepoints). (B–D) Phospho-kinetics of (B) phospho-BTK, (C) phospho-SYK, and (D) phospho-PLCγ2 stimulated with anti-IgM (25 µg/ml) for B cells pre-incubated either Fc (20 µg/ml) or CD52-Fc (20 µg/ml). (E–G) Phospho-protein comparison of BTK, PLCγ2 and Syk, 5 min post-stimulation of cells pre-treated with anti-Siglec10 blocking antibody (50 µg/ml) and CD52-Fc (20 µg/ml) or CD52-Fc alone. Unpaired two-tailed t-test used to compare groups. Data in (A–G) representative of at least n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.