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. 2021 Feb 4;11:626820. doi: 10.3389/fimmu.2020.626820

Figure 6.

Figure 6

Soluble CD52 reduces expression of surface immunoglobulin. (A) Bar plot of gene expression levels for the indicate gene. Data represent mean ± SD, * indicates DEG between CD52-Fc treated and vehicle control (Padj < 0.05) as determined by deseq2. (B) Healthy donor B cells were cultured for 7 days with vehicle control, Fc (20 µg/ml), or CD52-Fc (20 µg/ml) and the proportion of IgD+IgMlo anergic B cells among live naïve CD19+IgD+ B cells was quantified. (C) B cells were cultured with vehicle control, Fc (20 µg/ml), or CD52-Fc (20 µg/ml) and assessed for expression levels of surface IgM, IgD, and CD19 at indicated timepoints. Data expressed as MFI ratio relative to average vehicle control MFI. One-way analysis of variance was used in conjunction with Dunnett’s test for multiple comparisons to compare groups. Data in (B, C) representative of at least n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p <0.001, ****p < 0.0001.