Table 1.
Gene | Oligonucleotide Sequence (5ʹ–3ʹ) |
---|---|
IL-17a | Forward, CCTTCACTTTCAGGGTCGAG Reverse, CAGTTTGGGACCCCTTTACA |
iNOS | Forward, CCCTTCAATGGTTGGTACATGG Reverse, ACATTGACTCCGTGACAGCC |
COX-2 | Forward, TGCAGGCGAAGTGGGTTTTA Reverse, GAGTGGGAGGCACTTGCATT |
Fizz-1 | Forward, CGTGGAGAATAAGGTCAAGGA Reverse, CAGTAGCAGTCATCCCAGCA |
TNF-α | Forward, GCAAAGGGAGAGTGGTCAGG Reverse, TTTGTTCCACGGGGGTCTTG |
Arg-1 | Forward, CAGAAGAATGGAAGAGTCAG Reverse, CAGATATGCAGGGAGTCAC |
TGF-β1 | Forward, AGGAGACGGAATACAGGGCT Reverse, CCACGTAGTAGACGATGGGC |
IL1β | Forward, AAGCCTCGTGCTGTCGGACC, Reverse, TGAGGCCCAAGGCCACAGGT |
IL6 | Forward, CTCTGGGAAATCGTGGAAATG Reverse, AAGTGCATCATCGTTGTTCATACA |
MCP-1 | Forward, CTGCTAAGGACCACTTGCCA Reverse, CTCTGCCCTGTTTCCTTCGT |
YM1 | Forward, TCTCTACTCCTCAGAACCGTCAG Reverse, CGCATTTCCTTCACCAGAAC |
VEGFa | Forward, CACTTCCAGAAACACGACAAC Reverse, TGGAACCGGCATCTTTATCTC |
CXCL1 | Forward, GCCTCTAACCAGTTCCAGCA Reverse, TTGTCAGAAGCCAGCGTTCA |
Hes1 | Forward, ACACGACACCGGACAAACCA Reverse, TTATTCTTGCCCTTCGCCTCTT |
Cyclin D1 | Forward, GAGAAGTTGTGCATCTACAC Reverse, GAAGGGCTTCAACTGTTCC |
β-actin | Forward, GACGGCCAGGTCATCACTATTG Reverse, CCACAGGATTCCATACCCAAGA |
Abbreviations: IL-17a, interleukin 17a; iNOS, inducible nitric oxide; COX-2, cyclooxygenase 2; Fizz-1, found in inflammatory zone 1; TNF-α, tumor necrosis factor-α; Arg, Arginase-1; TGF-β1, transforming growth factor-β1; IL1β, interleukin 1β; IL6, interleukin 6; MCP-1, monocyte chemoattractant protein 1; YM1, chitinase3-like3; VEGFa, vascular endothelial growth factor a; CXCL1, chemokine ligand 1.