Fig. 5.

Hybrid NPs carrying antagomir-188 reverse aging-related bone loss. A) Representative micro-CT images of femora from the hybrid NPs, antagomir and hybrid NPs + antagomir groups. A sagittal section, a three-dimensional reconstruction of trabecular bone, and a horizontal section of cortical bone were presented in the left, upper right and bottom right, respectively. B) Representative images of FABP4 immunohistochemical staining of the distal femora, scale bar = 20 μm. C) Representative images of H&E staining of the distal femora. D) Quantification of FABP4 positive cells, scale bar = 20 μm. E) Quantification of bone lining cells. Statistic difference: *p < 0.05; **p < 0.01; ***p < 0.001.