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. 2021 Feb 6;20:675–687. doi: 10.1016/j.omtm.2021.02.002

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of the activation markers CD69⁺ and CD25⁺ by CD4⁺ and CD8⁺ populations after Dynabead or TransAct activation in a 24-well plate

CD69 and CD25 expression by T cells after 48-h activation stimulated by Dynabeads or TransAct, was measured using flow cytometry for donor 4 (▲), donor 5 (▸), donor 6 (), donor 7 (+), and donor 8 (★). An example of the flow cytometry gating is shown in (A) Fluorescence minus one (FMO) controls taken from donors 5/6 and full stain is the result from donor 5 isolated T cell TransAct cultures. CD69 and CD25 expression was measured for all starting material conditions (unsorted leukapheresis, biotin control, monocyte depletion, B cell depletion, and isolated T cells) where (B) CD4⁺ Dynabeads, (C) CD8⁺ Dynabeads, (D) CD4⁺ TransAct, and (E) CD8⁺ TransAct. A “not activated” control was included for comparison. In all cases, n = 5; error bars represent the mean ± 1 SD. The mean of the value is represented by □. Statistical significance was determined by one-way ANOVA and Dunnett’s hypothesis testing; ∗p < 0.05 and ∗∗p < 0.01 compared with unsorted leukapheresis products.

Inline graphic — Expression of the activation markers CD69⁺ and CD25⁺ by CD4⁺ and CD8⁺ populations after Dynabead or TransAct activation in a 24-well plate

CD69 and CD25 expression by T cells after 48-h activation stimulated by Dynabeads or TransAct, was measured using flow cytometry for donor 4 (▲), donor 5 (▸), donor 6 (), donor 7 (+), and donor 8 (★). An example of the flow cytometry gating is shown in (A) Fluorescence minus one (FMO) controls taken from donors 5/6 and full stain is the result from donor 5 isolated T cell TransAct cultures. CD69 and CD25 expression was measured for all starting material conditions (unsorted leukapheresis, biotin control, monocyte depletion, B cell depletion, and isolated T cells) where (B) CD4⁺ Dynabeads, (C) CD8⁺ Dynabeads, (D) CD4⁺ TransAct, and (E) CD8⁺ TransAct. A “not activated” control was included for comparison. In all cases, n = 5; error bars represent the mean ± 1 SD. The mean of the value is represented by □. Statistical significance was determined by one-way ANOVA and Dunnett’s hypothesis testing; ∗p < 0.05 and ∗∗p < 0.01 compared with unsorted leukapheresis products.