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. 2021 Feb 6;20:675–687. doi: 10.1016/j.omtm.2021.02.002

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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CAR retroviral transduction efficiency and growth curves for both activation reagents and tested conditions tested in a 24-well plate for donor 5 (▸), donor 6 (), donor 7 (+), and donor 8 (★)

(A) Example of flow cytometry gating for transduction efficiency (%TD). (B and C) (B) Transduction efficiency achieved after Dynabead activation and (C) transduction efficiency achieved after TransAct activation. n = 4, error bars represent the mean ± 1 SD. The mean of the value is represented by □. (D and E) Growth curves start after transduction reseeding (where 0 h since transduction is equivalent to 48 h after activation) for (D) Dynabead-activated and (E) TransAct-activated cultures.

Inline graphic — CAR retroviral transduction efficiency and growth curves for both activation reagents and tested conditions tested in a 24-well plate for donor 5 (▸), donor 6 (), donor 7 (+), and donor 8 (★)

(A) Example of flow cytometry gating for transduction efficiency (%TD). (B and C) (B) Transduction efficiency achieved after Dynabead activation and (C) transduction efficiency achieved after TransAct activation. n = 4, error bars represent the mean ± 1 SD. The mean of the value is represented by □. (D and E) Growth curves start after transduction reseeding (where 0 h since transduction is equivalent to 48 h after activation) for (D) Dynabead-activated and (E) TransAct-activated cultures.