Figure 6.

Efficiency of GFP/CAR retroviral transduction, cytotoxicity of CAR+ T cell products, and cytokine profiles

(A) Transduction efficiency (% TD) for donor 1 (◇)), donor 2 (○), donor 3 (⨯), donor 4 (▵), and donor 5 (▷), and CAR-transduced donor 2 (●), donor 4 (▴), donor 5 (▸), and donor 9 (★). (B) Target cell mediated cytotoxicity of CAR-transduced material for donors 2, 4, and 5 produced from starting material of unsorted leukapheresis, isolated T-cells and depleted of monocytes. The non-transduced and target cell only condition were included as controls. (C) Expression of cytokines at the end of a Raji cell-based cytotoxicity assay: tumor necrosis factor alpha (TNF-α), interleukin-2 (IL-2), granzyme B (GRANB), and interferon γ (INFγ). Error bars represent the mean ± 1 SD, where the mean of the value is represented by □. Statistical significance was determined by one-way ANOVA and Dunnett’s hypothesis testing; ∗p < 0.05 and ∗∗p < 0.01