Figure 7.

Overview of CAR T cell product memory phenotypes, CD4/CD8 ratios, and gene expression for CAR-transduced donors

(A) Review of memory phenotypes after expansion of CAR⁺ T-cells, measured by flow cytometry: CD45RA⁺CCR7⁺ (naive T [T_N] cells), CD45RA⁻CCR7⁺ (central memory T [T_CM] cells), and CD45RA⁻CCR7⁻ (effector memory T [T_EM] cells). (B) Ratio of CD4/CD8 T cells in final CAR T cell products. Error bars represent the mean ± 1 SD. (C) Differences in gene expression of the CAR T cell products at the end of production. Fold changes represent differences compared with unsorted leukapheresis products. Only the top 20 genes that vary under both processed conditions are shown for each condition. (D) Pathway scores for the activation and cytotoxicity gene set from the Nanostring data analysis. n = 3; the bar represents the median value and error bars the minimum and maximum values.