Table 1.

CD markers measured in flow cytometry to identify cell phenotype, activation, and transduction levels

Marker	Purpose	Literature values (%)	Donor range (%)	Purified condition ranges (%)
				Isolated CD3 T cells	CD14 depletion	CD19 depletion	Biotin control
CD235a	Expressed by red blood cells	–	2.2–15	0.07–2.4	2.9–19	2.4–16	2.8–6.6
CD45	Expressed by all WBCs	–	83–96	98–99	79–95	82–97	85–97
CD3	Pan T cell marker, expressed by all T cells	44–8422	25–63	81–97	57–75	28–72	25–64
CD4	A subset of CD3+ T cells, T helper cells	23–5922	53–81	53–81	53–81	53–81	53–81
CD8	A subset of CD3+ T cells, cytotoxic T cells	10–4322	13–35	13–35	13–35	13–35	13–35
CD14	Expressed by monocytes	8.0–4523	9.3–32	0.1–0.7	0.1–1.1	12–28	12–27
CD19	Expressed by B cells	4.4–2822	2.3–13	0.3–1.3	5.3–18	0.05–0.13	2.1–15
CD56	Natural killer (NK) cells and a subset of CD3+ T cells (NK T cells)	3.7– 4.622	2.6–8.9	0.6–1.5	3.6–8.5	3.7–6.2	3.5–5.7