Figure EV2. Experimental detail of in vivo killing assay.

1. Timeline of in vivo killing protocol, and diagram of pulse‐labelling of CD45.1 splenocytes with different concentrations of N₃₉₆ peptide and staining with cell trace violet.
2. Weights of WT and KO mice infected with LCMV which received pulsed‐labelled splenocytes 8dpi for in vivo killing experiment shown in Fig 4A.
3. Weights of WT and KO mice infected with LCMV then passively transferred with KL53 that received pulsed‐labelled splenocytes 8dpi for in vivo killing experiment shown in Fig 4B.

Data information: All data are presented as mean with standard error (n = 6). **P < 0.01, unpaired t‐test.