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. 2020 Dec 1;40(5):e106228. doi: 10.15252/embj.2020106228

Figure 3. TRIM21 uses anti‐N antibodies to induce an N‐specific CTL response.

Figure 3

  1. CD8 T cells in WT mice were depleted by administration of anti‐CD8 mAb 1 day prior to infection with 105 FFU LCMV. Anti‐N mAb KL53 or PBS control was passively transferred IP on days 1 and 3pi. Viral titres in the spleen, lung, liver and kidney of all mice were determined by FFA day 8pi.
  2. Spleens from WT and KO mice +/‐ mAb KL53 day 8pi with 0.5 × 105 FFU LCMV were analysed for the presence of LCMV N‐specific CTLs by staining with the class I N396‐404 tetramer.
  3. Spleens from naïve WT and KO mice day 10pi with 0.5 × 105 FFU LCMV were stained with N tetramer.
  4. Macrophages were depleted in WT mice by administration of clodronate liposomes 1 day prior to infection with 0.5 × 105 FFU LCMV. KL53 was administered on days 1 and 3pi, and N396‐specific CTLs in the spleen were measured day 8pi by tetramer staining.

Data information: Each data point represents one mouse from the same experiment. Horizontal bars on each graph correspond to the mean, **P < 0.01, ***P < 0.001, NS not significant, unpaired t‐test.