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. 2021 Feb 8;40(5):e107037. doi: 10.15252/embj.2020107037

Figure 3. Pol δ facilitates bypass of a leading‐strand Tg.

Figure 3

  1. Outline of pulse chase reaction used in (B–E).
  2. Pulse chase reactions on the Tg template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel.
  3. Schematic of stall and bypass products generated by SwaI and BamHI cleavage (D and E).
  4. Pulse chase reaction on the Tg template in the absence and presence of 10 nM Pol δ analysed on a urea polyacrylamide gel.
  5. Titration of Pol δ in a pulse chase reaction on the Tg template. Pol δ was added after the 0 min time point was taken.
  6. Quantification of bypass 5 min after addition of the chase for the data in (E).
  7. Titration of Pol δ or Pol ε (1, 2.5, 5, 7.5, 10 nM) into a primer extension assay on a Tg template in the presence of PCNA and RFC. The primer/template sequence and location of the lesion are shown above.