Skip to main content
. 2021 Feb 8;40(5):e107037. doi: 10.15252/embj.2020107037

Figure EV3. (Related to Fig 3). Analysis of Tg bypass by Pol δ and Pol ε.

Figure EV3

  1. Potassium glutamate titration into primer extension reactions on the Tg template shown in Fig 3G in the presence of Pol δ (1 nM), RFC, and PCNA.
  2. Pol δ titration (1, 2.5, 5, 7.5, 10 nM) into primer extension reactions on the Tg template shown in Fig 3G in the absence and presence of RFC and PCNA.
  3. Pol ε titration (0.5, 1, 2, 4, 8 nM) into primer extension reactions on the Tg template in the absence and presence of RFC and PCNA.
  4. Pol ε titration (0.5, 1, 2, 4, 8 nM) into primer extension reactions on the Tg template with either a 20 nt or 30 nt primer annealed in the presence of RFC and PCNA.
  5. Titration of Pol ε and Pol εexo− (1, 2, 4, 8, 16 nM) into primer extension reactions on the Tg template in the presence of RFC and PCNA.