Figure 4. Pol δ likely performs insertion and extension during Tg bypass.

1. Schematic showing insertion and extension at the Tg site in the sequence context used in replication assays.
2. Mapping the 3′‐end of the stalled nascent leading strand. Pulse chase reaction on Tg and CPD templates. Where present, Pol δ (10 nM) was added immediately after the 0 min time point. Products were cleaved with SwaI and BamHI and resolved alongside a sequencing ladder corresponding to the nascent strand. dd; dideoxy.