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. 2021 Feb 8;40(5):e107037. doi: 10.15252/embj.2020107037

Figure 6. The replisome bypasses 8oxoG by a replicase switch mechanism.

Figure 6

  1. Standard replication assay on undamaged (UD), 8‐oxoguanine (8oxoG), and thymine glycol (Tg) templates in the absence of TLS components.
  2. Pulse chase reaction on UD and 8oxoG templates. Where present, Pol δcat (10 nM) was added directly after addition of the chase.
  3. Pulse chase reactions on the 8oxoG template in the absence and presence of 10 nM Pol δ analysed on a denaturing gel.