Table 1.
Statistics for Cryo‐EM single particle image analysis and structure refinement.
| Cryo‐EM density map | CA‐CCCT (4.5Å) (EMDB‐30239) (PDB 7BY0) | Asymmetric CA‐CCCT‐CNNT (EMDB‐30241) | CA‐CCpep‐CNNT (EMDB‐30237) (PDB 7BXT) | CA‐CCCT (6.78Å) (EMDB‐30240) |
|---|---|---|---|---|
| Data collection and processing | ||||
| Sample | CA‐CCCT‐CNNT complex | CA‐CCpep‐CNNT complex | CA‐CCCTcomplex | |
| Magnification | 50,000 | 50,000 | 50,000 | 50,000 |
| Voltage (kV) | 200 | 200 | 200 | 200 |
| Electron exposure (e–/Å2 ) | 100 | 100 | 100 | 100 |
| Defocus range (µm) | −0.3 (−7.0) | −0.3 (−7.0) | −0.3 (−7.0) | −0.3 (−7.0) |
| Pixel size (Å) | 1.10 | 1.10 | 1.47 | 1.09 |
| Symmetry | C2 | C1 | C2 | C2 |
| Initial particle images (n) | 3,385,783 | 3,385,783 | 4,192,300 | 3,423,115 |
| Final particle images (n) | 38,542 | 3,959 | 118,294 | 39,542 |
| Map resolution (Å) | 4.5 | 7.8 | 4.2 | 6.78 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Refinement | ||||
| CCmap_model | 0.87 | 0.84 | ||
| Model compositions | ||||
| Nonhydrogen atoms | 12,693 | 15,715 | ||
| Protein residues | 872 | 1,204 | ||
| Nucleic acids | 285 | 290 | ||
| R.m.s. deviations | ||||
| Bond lengths (Å) | 0.007 | 0.010 | ||
| Angles (°) | 0.939 | 1.169 | ||
| Ramachandran plot | ||||
| Favored (%) | 96.79 | 93.36 | ||
| Allowed (%) | 3.21 | 6.64 | ||
| Outliers (%) | 0.00 | 0.00 | ||
| Model validation | ||||
| Rotamer outliers (%) | 0.15 | 0.39 | ||
| Clash score | 11.07 | 9.69 | ||
| Cβ outliers (%) | 0.00 | 0.00 | ||
| CaBLAM outliers (%) | 2.55 | 3.93 | ||
FSC, Fourier shell correlation; R.m.s. deviations, root‐mean‐square deviations.