Figure 2. IFT‐A and IFT‐B proteins accumulate at ciliary tip in the IFT54^Δ261–275 mutant while at proximal end of cilia in IFT54^Δ342–356 mutant.

• A
  Immunostaining of IFT54 mutated proteins in various deletion mutants as indicated; wild‐type (WT) cells were used as control. Specimens were stained with anti‐HA and anti‐α‐tubulin antibodies followed by imaging using an epifluorescence microscope. Arrows indicate protein accumulation at ciliary tip (left) or proximal end of cilia (right). Scale bars, 5 µm.
• B, C
  Immunostaining analysis of IFT43 and IFT38 in the deletion mutants as indicated. Cells were fixed and stained with antibodies against IFT43 (an IFT‐A subunit) (B) or against IFT38 (an IFT‐B subunit) (C) followed by imaging using both DIC and epifluorescence microscopy. IFT43 and IFT38 accumulated at the ciliary tip in IFT54^Δ261–275 mutant but at proximal end of cilia in IFT54^Δ342–356 mutant. Arrows indicate ciliary bulges. WT, wild‐type cells. Scale bars, 5 µm.