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. 2021 Feb 12;10(2):409. doi: 10.3390/foods10020409

Table 1.

Buffers and their composition used to extract proteins from Pacific oyster.

Buffer pH Composition
Citrate-3 3.0 Citric acid 0.082 M Trisodium citrate 0.018 M
Citrate-5 5.0 Citric acid 0.065 M Trisodium citrate 0.035 M
TBS 7.4 Tris 25 mM Potassium Chloride 3.0 mM Sodium Chloride 140 mM
TBSN 7.4 Tris 25 mM Potassium Chloride 3.0 mM Sodium Chloride 1 M
PBS 7.4 Phosphate 10 mM Potassium Chloride 2.7 mM Sodium Chloride 137 mM
PBSN 7.4 Phosphate 10 mM Potassium Chloride 2.7 mM Sodium Chloride 1 M
Carbonate-9 9.2 Sodium Carbonate 0.01 M Sodium Bicarbonate 0.09 M
Carbonate-10 10.3 Sodium Carbonate 0.07 M Sodium Bicarbonate 0.03 M