Table 4.

Proteins identified using LC-MS in the SDS-PAGE bands corresponding to the IgE-reactive bands. The top three proteins from Mascot search engine result in each band are presented and ordered based on their abundance in the spot.

Band No	Protein	Accession ID	Exp MW	Theo MW	Mascot Score	Coverage (%)	Number of Significant Peptides	emPAI
Raw
1	Tropomyosin	B7XC66_CRAGI	36	33	1566	62	19	22.48
	Myosin heavy chain	K1RSS3_CRAGI		230	1463	20	33	1.42
	Filamin	K1PW06_CRAGI		326	553	12	6	0.23
2	Arginine kinase	K1PLF9_CRAGI	39	40	1749	72	23	34.84
	Tropomyosin	B7XC66_CRAGI		33	1601	55	19	20.33
	Filamin	K1PW06_CRAGI		326	2533	32	65	1.17
3	Retinal dehydrogenase I	K1QVG5_CRAGI	50	53	1256	59	43	6.72
	Aldehyde dehydrogenase	K1QNT7_CRAGI		58	648	42	17	3.01
	Tubulin α-1C chain	K1QII6_CRAGI		51	744	47	14	2.74
4	Paramyosin	K1QTC1_CRAGI	100	98	6288	74	69	61.54
	Alpha-actinin	K1RH58_CRAGI		102	1480	56	38	4.44
	Filamin	K1PW06_CRAGI		326	1711	28	50	1.00
5	Filamin	K1PW06_CRAGI	150	326	7159	61	151	7.06
	Clathrin heavy chain	K1PNR3_CRAGI		193	1660	44	60	2.22
	Spectrin α chain	K1R401_CRAGI		287	1905	45	77	1.69
Heated
6	Tropomyosin	B7XC66_CRAGI	36	33	1570	59	21	33.41
	Myosin heavy chain	K1RSS3_CRAGI		230	1988	27	44	1.56
	Filamin	K1PW06_CRAGI		326	635	16	23	0.32
7	Tropomyosin	B7XC66_CRAGI	39	33	3535	57	21	60.09
	Troponin T	K1QPC9_CRAGI		21	522	74	10	7.17
	Non-neuronal cytoplasmic intermediate filament protein	K1PBC0_CRAGI		70	410	35	15	1.19
8	Tropomyosin	B7XC66_CRAGI	50	33.1	822	52	13	5.77
	Non-neuronal cytoplasmic intermediate filament protein	K1PBC0_CRAGI		69.6	1599	49	29	5.32
	Adipophilin	K1PJC1_CRAGI		54.4	791	41	16	2.44
9	Paramyosin	K1QTC1_CRAGI	100	98	7125	63	46	20.17
	Filamin	K1PW06_CRAGI		326	5441	47	107	2.60
	Myosin heavy chain	K1RSS3_CRAGI		230	4644	45	115	1.71
10	Myosin heavy chain	K1R1B3_CRAGI	120	80	3998	58	36	11.60
	Paramyosin	K1QTC1_CRAGI		98.1	1523	45	36	2.15
	Filamin	K1PW06_CRAGI		326.2	1796	23	53	0.73