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. 2021 Feb 12;22(4):1828. doi: 10.3390/ijms22041828

Figure 4.

Figure 4

Inactivation of the JNK pathway by atorvastatin regulates PAI-1 production and monocyte migration. (A) Western blot analysis of PAI-1 expression and JNK activation in control (Con), atorvastatin-treated (Atorva), irradiated (IR), and IR + Atorva HUVECs in the presence or absence of anisomycin (Ani, 50 nM). (B) mRNA levels of PAI-1 and (C) immunofluorescence images and the fluorescence intensity of phosphorylated c-Jun (green) in Con, Atorva, IR, and IR + Atorva HUVECs in the presence or absence of Ani. (D) Trans-endothelial migration of THP-1 cells in atorvastatin-treated irradiated HUVECs. Representative images of migrated carbosyfluorescein diacetate succinimidyl ester (CFSE)-stained THP-1 cells (green). The number of migrated THP-1 cells was quantified and plotted (lower). Data are presented as the mean of ± standard deviation of triplicate experiments. Images are representative of 3 independent experiments. * p < 0.05 vs. negative control (Con), # p < 0.5 vs. irradiated control (IR). Scale bars represent 100 μm.