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. 2021 Feb 13;22(4):1861. doi: 10.3390/ijms22041861

Figure 5.

Figure 5

Silencing Fosl-2 suppresses autophagy in cardiac fibroblasts. Fosl-2 silencing was performed in cardiac fibroblasts using Fosl-2 antisense oligonucleotide GapmeR (Fosl-2 knockdown: k.o.) and was compared to scrambled control (Scr). Cells were cultured in starvation medium (1% FBS), unless otherwise indicated. (A) Fosl2 gene expression (n = 5, Mann–Whitney U test), (B) representative immunoblots and densitometric analysis of Fosl-2 protein levels (n = 7, Mann–Whitney U test), (C) expression of genes encoding αSMA (Acta2) and type I collagen (Col1a1) compared to Scr control (n = 5, Mann–Whitney U test). (D) pro-collagen I levels in supernatants (n = 5, unpaired t-test), (E) representative immunoblots and densitometric analysis of αSMA protein levels (n = 4, Mann–Whitney U test). (F) Representative immunofluorescence staining of αSMA (n = 4, nuclei in blue are stained with DAPI, scale bars: 10 µm), (G) representative immunofluorescence staining of LC3B (n = 5, TGF-β treatment 24 h) and (H) densitometry analysis of Becn1 and LC3B II protein levels presented in relation to the respective Scr controls (n = 6, Mann–Whitney U test vs. Scr control).