Skip to main content
. 2021 Feb 12;22(4):1858. doi: 10.3390/ijms22041858

Figure 1.

Figure 1

CRISPR/Cas 9 knocked down the δ-ENaC subunit in the A549. (A) Upper panel, A549 cell lysate blotted against anti δ-ENaC and β-Actin antibodies. Representative western blot out of three independent biological replicates is shown. A549 cells transfected with either CRISPR/Cas 9 control plasmid (control) or CRISPR/Cas 9 δ-ENaC KO plasmid (KD). The lower panel; mean values of quantitation of three western blots is shown. (B) Upper panel, representative confocal microscope image of anti δ-ENaC antibody, is shown. A549 cells transfected with either CRISPR/Cas 9 control plasmid (control) or CRISPR/Cas 9 δ-ENaC KO plasmid (KD). The lower panel; mean values of quantitation of 7 immunofluorescence images are shown. Significant differences are indicated, * p < 0.5, unpaired Student’s t-test, n = 5–7.