Figure 3.
The effects of complexes 1, 2 and 3 on apoptosis/necrosis in CHO-K1 (A) and HepG2 cells (B) determined by flow cytometry. The cells (2 × 104/well) were exposed to 50 μM/L of each compound for 24 h. In parallel, the cells were incubated in the same conditions with addition of comparator salts: CoCl2 (comparator for cobalt complexes 1 and 2) and VOSO4 (comparator for vanadium complex 3). Note that complex 3 was the most active promotor of apoptosis ((B), p < 0.001 vs. control) while complexes 1 and 2 caused mainly necrotic cell death ((B), for 1 and 2 p < 0.01 vs. control). Apoptosis was determined by flow cytometry with Annexin V/EthD-III double staining and DAPI as a control of viable cells. Three experiments were performed with similar results and the bars represent arithmetic mean values and standard deviation (M; ±SD), n = 3.