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Glycolysis and redox balance in two-dimensional (2D) and 3D culture. Purine, a nucleotide, is biosynthesized using ribose 5-phosphate, which is generated in the pentose phosphate pathway (PPP). The levels of the intermediates of glycolysis were significantly lower in 2D than in 3D. (* p < 0.05, ** p < 0.01, *** p < 0.001).
