Table 3.
The main parameters of the calcium activity of primary hippocampal cultures under the influence of ischemic factors together with the blockade of intracellular kinases.
| Group | Active Cells, % 1 | Frequency, osc/min | Duration, s |
|---|---|---|---|
| Sham | 61.13 ± 4.21 | 1.43 ± 0.27 | 11.79 ± 0.88 |
| GD control | 47.17 ± 7.05 * | 1.04 ± 0.25 | 11.77 ± 0.67 |
| Hypoxia control | 35.91 ± 1.05 * | 0.48 ± 0.12 * | 11.95 ± 0.36 |
| GD + SRC | 51.97 ± 5.79 | 1.03 ± 0.41 | 11.44 ± 0.705 |
| Hypoxia + SRC | 35.87 ± 5.67 * | 1.03 ± 0.41 | 11.35 ± 0.07 # |
| GD + Ikkb | 21.54 ± 4.58 *,# | 0.62 ± 0.11 *,# | 15.93 ± 1.69 *,# |
| Hypoxia + Ikkb | 38.69 ± 6.58 * | 1.17 ± 0.21 @ | 11.08 ± 1.03 |
| GD + eEF2K | 18.41 ± 2.55 *,# | 0.81 ± 0.13 * | 17.67 ± 2.68 *,# |
| Hypoxia + eEF2K | 10.67 ± 2.05 *,@ | 1.15 ± 0.29 @ | 9.38 ± 0.62 @ |
| GD + FLT4 (VEGFR3) | 67.74 ± 9.33 # | 0.58 ± 0.08 *,# | 8.94 ± 0.79 *,# |
| Hypoxia + FLT4 (VEGFR3) | 63.58 ± 5.15 @ | 0.87 ± 0.11 @ | 11.66 ± 1.83 |
1 Active cells, %—the cell number with at least one recorded oscillation divided by the total cell number; * versus “Sham”, p < 0.05; # versus “Control GD”, p < 0.05; @ versus “Control Hypoxia”, p < 0.05, two-way ANOVA and Tukey post hoc test.