Table 1.
Semi-quantitative IHC analysis.
| Staining Intensity Score (0–3) | |
| 0 | No staining |
| 1 | Weak staining |
| 2 | Moderate staining |
| 3 | Intense staining |
| Percentage of stained cells score (0–4) | |
| 0 | 0% |
| 1 | 1–4% |
| 2 | 5–9% |
| 3 | 10–49% |
| 4 | ≥50% |
| Combination Score = % score (0–4) x intensity score (0–3) | |
| negative | 0–3 |
| positive | ≥4 |
A modified quick score was used for semi-quantification of tumor cells (TC) and immune cells (IC) within the two different tumor regions (MIBC and carcinoma in situ). The product of both scores (intensity and proportion) was used to indicate the level of protein expression. Protein expression was considered negative when the combination IHC score was ≤3 and positive when 4 or greater.