Figure 1.

The mitogenic activity of vascular endothelial growth factor VEGF-A₁₆₅ and fibroblast growth factor FGF-2M diluted in the culture medium. The adipose tissue-derived stem cells (ADSCs) (A,B) or human umbilical vein endothelial cells (HUVECs) (C,D) were grown in media enriched with commercial VEGF-A₁₆₅ (VEGF com.) or our recombinant VEGF-A₁₆₅ in concentrations from 10 to 1000 ng/mL (A,C); in media enriched with commercial FGF-2 (FGF-2 com.) or our recombinant FGF-2M in concentrations from 5 to 250 ng/mL (B,D). The growth factors were added into Dulbecco’s modified Eagle medium (DMEM) with 10% fetal bovine serum (FBS) for ADSCs (A,B), and into EGM2-weak for HUVECs (C,D). Control cells were grown in media without growth factors (Ctrl). The cell number was determined on days 1, 3, and 7 after seeding. Mean ± standard deviation (SD) from 3 wells. Holm–Sidak method, p ≤ 0.05. The samples were statistically compared on the indicated day after seeding. Statistically significant differences are depicted above the columns. * statistically significant difference versus control sample (Ctrl). # statistically significant difference versus sample containing a corresponding concentration of commercial growth factor.