Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 12;22(4):1843. doi: 10.3390/ijms22041843

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The effect of medium exchange on the mitogenic activity of VEGF-A₁₆₅ diluted in the culture medium. The HUVECs were grown in media enriched with commercial VEGF-A₁₆₅ (VEGF com.) or our recombinant VEGF-A₁₆₅ in the concentration range from 10 to 1000 ng/mL. VEGF-A₁₆₅ was added into EGM2-weak. Control cells were grown in media without growth factors (Ctrl). The cell number was determined on days 1, 3, and 7 after seeding. In some of the samples, the medium containing the corresponding concentration of growth factor was exchanged for a fresh one on day 3 after cell seeding. Mean ± SD from 3 wells. Holm–Sidak method, p ≤ 0.05. The samples were statistically compared on the indicated day after seeding. Statistically significant differences are depicted above the columns. * statistically significant difference versus control sample (Ctrl). # statistically significant difference versus sample containing the corresponding concentration of commercial growth factor. × statistically significant difference versus sample containing the corresponding concentration of growth factor without medium exchange.