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. 2021 Feb 13;10(2):202. doi: 10.3390/pathogens10020202

Figure 4.

Figure 4

TPP inhibits the NF-κB signaling pathway in PRRSV-infected Marc-145 cells. (A) Marc-145 cells were infected with PRRSV-CHR6 (MOI = 0.6) in the presence or absence of TPP, and the mRNA level of p65 was assessed by qRT-PCR. (B) Marc-145 cells were infected with PRRSV-EGFP (MOI = 0.6) in the presence or absence of TPP, and immunofluorescence assay (IFA) for the p65 protein was performed at 36 h post-infection (hpi) using Alexa-Fluor-555-conjugated anti-rabbit secondary antibody (red). Nuclei were stained with DAPI (blue). Scale bar, 20 μm. LPS served as a positive control. Data are representative of the results of three independent experiments (means ± SE). Significant differences compared to the control group are denoted by ** p < 0.01.