Figure 5.

Expression of cytokines in Marc-145 cells treated with TPP. (A–D) Marc-145 cells were mock-infected or infected with PRRSV-CHR6 (MOI = 0.6) in the presence or absence of TPP (50 μg/mL) for 12 h. The expression of pro-inflammatory cytokines IFN-β (A), IL-6 (B), IL-8 (C), and TNF-α (D) was analyzed using qRT-PCR. Relative expression (fold) in comparison with mock-infected cells (set up as 1) is shown. LPS served as a positive control. Data are the results of three independent experiments (means ± SE). Significant differences between PRRSV-infected cells and those treated with TPP are denoted by * p < 0.05, ** p < 0.01, and *** p < 0.001.