Table 1.
Primer a | Sequence (5′–3′) b |
---|---|
N-F | AAAACCAGTCCAGAGGCAAG |
N-R | CGGATCAGACGCACAGTATG |
GAPDH-F | TGACAACAGCCTCAAGATCG |
GAPDH-R | GTCTTCTGGGTGGCAGTGAT |
P65-F | AGAGCCTCCTGCACCAGTTCT |
P65-R | -R TCACTCCTTCTTCCTG |
IFN-β-F | GCAATTGAATGGAAGGCTTGA |
IFN-β-R | CAGCGTCCTCCTTCTGGAACT |
IL-6-F | AGAGGCACTGGCAGAAAAC |
IL-6-R | TGCAGGAACTGGATCAGGAC |
IL-8-F | CACTGTGAAAATTCAGAAATCATTGTTA |
IL-8-R | CTTCACAAATACCTGCACAACCTTC |
TNF-α-F | TCTGTCTGCTGCACTTTGGAGTGA |
TNF-α-R | TTGAGGGTTTGCTACAACATGGGC |
a F, forward primer; R, reverse primer. b Green monkey gene sequences and PRRSV gene sequences were downloaded from GenBank.