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Localization of hypoxia inducible factor (HIF)-1α, α-smooth muscle actin (α-SMA) + cells and mac387+ macrophages in the aortic wall. Double-immunostaining for HIF-1α and α-SMA in 0 h (A–D) and 3 h (E–H). The white arrows indicate the co-localized region. Double-immunostaining for HIF-1α and mac387+ macrophages in 0 h (I–L) and 6 h (M–P). Scale bar = 15 µm. 0 h (n = 5), 3 h (n = 5), and 6 h (n = 5).
