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. 2021 Feb 11;13(4):755. doi: 10.3390/cancers13040755

Table 1.

The six tumor dissociation methods evaluated using the CyTOF panel.

Dissociations/
Conditions		 Primary Enzyme Duration Additional Enzyme Duration

  1. Coll

 Collagenase II + CaCl2 2 h
  • 2.

    Coll+Try

 Collagenase II + CaCl2 2 h TrypLE 5 min
  • 3.

    Mil 1 h

 Miltenyi 1 h
  • 4.

    Mil 2 h

 Miltenyi 2 h
  • 5.

    Coll+Dis

 Collagenase II + CaCl2 1 h Dispase 30 min
  • 6.

    Mech

 Mechanical. − No enzyme added. - - -