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. 2021 Feb 14;10(2):366. doi: 10.3390/plants10020366

Figure 3.

Figure 3

(a) Human visceral adipose-derived stem cells (vASCs) were treated with the resveratrol (Res) and piceatannol (Pic) in the absence or presence of adipogenic differentiation medium (ADM) for 14 days and stained with oil red O (ORO). Intracellular lipid accumulation levels were quantified by extracting ORO-stained lipid droplets with 100% isopropanol and the absorbance was measured at 495 nm. Data are expressed as means ± SD from three independent experiments (** p < 0.01 and *** p < 0.001 vs. ADM-only-treated group of the Res treatment groups; # p < 0.05 and ### p < 0.001 vs. ADM-only-treated group of the Pic treatment groups; ᶲᶲ p < 0.01). (b,c) Photographs are representative images of the three independent experiments (magnification 10× and 40×). (d) vASCs were treated with Res and Pic in the presence of ADM for 14 days and stained with 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY). Confocal images of fat body stained with BODIPY 493/503, Phalloidin, and 4′,6-diamidino-2-phenylindole (DAPI) to show lipid droplets, actin filaments, and nuclei, respectively.