Skip to main content
. 2021 Feb 14;10(2):393. doi: 10.3390/cells10020393

Table 1.

Microalgae cultivation correlation with nitrogen content.

Microalgae Types Experiment Outcomes Growth and Biochemical Composition Reference
Nannochloropsis sp. Transferred from nitrogen-sufficient to -deficient condition Lipid content increased from 32% to 60% [27]
Chlorella vulgaris Lipid production under nitrogen starvation conditions Lipid productivity was 58.39 mg/L/day. [28]
Chlorella zofingiensis 10 days cultivation in two conditions: nitrogen starvation and nitrogen repletion (0 g/L and 1.1 g/L, respectively) Rapid growth in nitrogen-sufficient culture medium, whereas growth inhibition under nitrogen starvation condition [3]
Scenedesmus sp. Effects of nitrogen limitation and starvation on morphological and biochemical changes Nitrate starvation generates 27% lipid accumulation [29]
Nannochloropsis oceanica 14 days under nitrogen-deplete and nitrogen-replete conditions Regulation of metabolic pathways along with acetyl CoA synthesis and lipid degradation for TAG synthesis [30]
Chlorella sp. and Nannochloropsis oculata 13 days in Conway nutrient batch cultures without nitrogen in the 7th day of growth 15.3 ±1.0% lipid in Chlorella sp., 33.7 ± 2.8% lipid in N. oculata under nitrogen limitation [31]
Phaeodactylum tricornutum Lipid accumulation in nitrogen-deficient medium High lipid content (53.04 ± 3.26%) [5]
C. reinhardtii Effect of different nutrients on standard TAP, TAP nitrogen deficiency (T-N), TAP without nitrogen and phosphorus (T-N-P), TAP nitrogen deficiency with additional phosphorus 1 M(K)PO4 (T-N+P) on growth and lipid accumulation Lipid contents in cells increased as a result of nitrogen-deficient conditions; the highest lipid content was 104.7% in T-N-P condition and the lowest lipid values were 49.9% in T-N condition [32]
I. galbana Effect of different nitrogen concentrations(0, 36, 72, 144 and 288 mg/L) on the growth rate and biochemical composition Growth decreased in nitrogen-deficient conditions; in contrast, carbohydrates and fatty acids showed the highest value, 47% and 75%, respectively [7]
Scenedesmus obliquus Various nitrogen concentrations’ effect on growth and lipid production Lipid production increased with increasing nitrogen concentration; the highest cell density (1.7 × 107 cells/mL) and lipid production (242.4 mg/L) [33]