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Time-of-addition assay. Vero E6 cells were infected with SARS-CoV-2 (BavPat1 isolate) at an MOI of 1. (A) Schematic layout of the assay. 20 µM berberine (BBR), 0.25 µM obatoclax (OLX), or 0.1% DMSO as a control was added to the infected cells at the indicated time points. (B) Plaque assay titers from cell culture supernatants that were collected at 10 hpi. Bars and error bars represent means and SD of n = 2 replicates.
