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. 2021 Feb 11;13(2):282. doi: 10.3390/v13020282

Figure 6.

Figure 6

Berberine and obatoclax inhibit SARS-CoV-2 replication in primary nasal epithelial cells. Primary nasal epithelial cells cultured on an air-liquid interface were infected with SARS-CoV-2 (BavPat1 isolate) at an MOI of 10 in the presence of the indicated concentrations of (A) berberine (BBR) or (B) obatoclax (OLX) in a two-fold dilution series or 0.1% DMSO as a control. Viral RNA from duplicate apical surface supernatants at 72 hpi was quantified by qRT-PCR and plotted as percentage inhibition compared to the DMSO control. Error bars indicate SD (n = 2). Dashed line indicates 50% inhibition. Primary nasal epithelial cells cultured on an air-liquid interface were treated with the indicated concentrations of (C) BBR or (D) OLX or 0.1% DMSO. At 72 h, the ATP content in cells from duplicate samples was measured as a measure of cell viability, plotted as a percentage of the DMSO control. Error bars indicate SD. Dashed lines indicate 50% viability. A representative of two independent experiments is shown. The shaded grey box indicates that different OLX concentrations were used for antiviral (B) and viability assays (D).