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. 2021 Feb 11;22(4):1796. doi: 10.3390/ijms22041796

Figure 2.

Figure 2

(A) Relative mRNA expression of Ube2d2, Ube2e1, Rbx1, Uhrf2, Trim9, Birc3, Xaf1, Kdm2b, Siah2, Ube3b, and Senp1 in NSC-34 cells expressing SOD1WT or SOD1A4V in presence of 1 mM of H2O2 for 3 h. (B) Western blotting of SOD1-GFP proteins in NSC-34 cells expressing SOD1WT-GFP, SOD1A4V-GFP, SOD1V31A-GFP, and SOD1G93C-GFP using antibodies against GFP or Gapdh. (C) Confocal microscopy analysis of SOD1WT–GFP or SOD1mutant-GFP in NSC-34 showing aggregates (48 h post-transfection). Scale bar: 30 µm. (D) Relative mRNA expression of Uhr2 in NSC-34 cells expressing SOD1WT, SOD1A4V, SOD1V31A, and SOD1G93C in absence or presence of 1 mM of H2O2 for 3 h. * p < 0.05.