Figure 9.

Growth of wild type and sigH mutant strain after treatment with nisin. C. difficile 630∆erm (WT, light colors, squares) and a sigH mutant strain (ΔsigH, dark colors, triangles) were grown at 37 °C in brain heart infusion (BHI) in an anaerobic chamber. In the exponential growth phase cultures were either treated with nisin (shades of orange) or left untreated (control, shades of blue). Error bars represent standard deviations of the optical densities determined in three biological replicates.