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. 2021 Feb 11;22(4):1801. doi: 10.3390/ijms22041801

Figure 2.

Figure 2

(A,B) A375 and A375-BIR cells were transiently transfected with 40 nM of the miR-181a and -181b mimic or inhibitor or the appropriate controls and assayed for proliferation by the MTT viability assay. Data are expressed in terms of percentage of cell viability as compared to untransfected cells. Each value represents the arithmetic mean of three independent experiments performed with triplicate samples. *** p < 0.001. (C,D) M14 and M14-BIR cells were transiently transfected with 40 nM of the miR-181a and -181b mimic or inhibitor or the appropriate controls and assayed for proliferation by the MTT assay. Data are expressed in terms of percentage of cell viability as compared to untransfected cells. Each value represents the arithmetic mean of three independent experiments performed with triplicate samples. *** p < 0.001. (E–G) Colony formation assays were performed to determine the colony formation ability of A375 and A375-BIR melanoma cells transfected with miR-181a and -b mimics or inhibitor or the appropriate controls. Representative images and quantification of visible colonies have been presented. The colony number in the DMSO-treated group was set as 100%. All the experiments were performed in triplicate and the relative colony formation rates are shown as the mean +/− SD. *** p < 0.001; **** p < 0.0001; ns, not significant. H–I) Effect of miRNA-181a and b forced expression or inhibition on CASP-9 activation in A375 and A375-BIR cells. The determination of CASP-9 activity was carried out by using Caspase-Glo® 9 assay. Data are expressed in fold change relative to vehicle control +/− SD of three independent assays with 3 replicates for each one. *** p < 0.001 and ** p < 0.01 versus vehicle control; ns, not significant.